Tuesday, December 24, 2019

The Most Dangerous Game And Porrphyrias Lover Analysis

Isnt it crazy that some people can be so insane that they think that murder is okay? Believe it or not, in some cases this is what people believe. Two examples of this are the stories, The Most Dangerous Game, and Porphyrias Lover where the authors clearly show this satanic message. Through the use of characterization, and detail the authors Connell, and Browning contend that in some minds people believe murder is acceptable. Both stories, The Most Dangerous Game, and Porphyria’s Lover use characterization to convey how psycho the characters are. General Zaroff is portrayed to be delusional and crazy because of the fact that he is a murderer. At one point General Zaroff exclaims†¦ We try to be civilized here. Zaroff says that he is†¦show more content†¦Zaroff is proven crazy through the use of detail when he describes killing humans as hunting. Why should I not be serious? I am speaking of hunting. Zaroff had gotten bored of hunting animals because they were no t able to reason, which led him to try hunting humans which is murder. In his mind he sees no wrong in killing people and is astonished when Rainsford bashes on his idea of the new hunt. The Poem Porphyria’s Lover also used detail to show how the characters ideas and beliefs are different than others and that he doesnt see the wrong in murder. In stanza 60 he says†¦ And thus we sit together now, And all night long we have not stirred, And yet God has not said a word! He thinks that because god has not showed a sign that killing a person is wrong, that no wrong is being done. Again the authors show the idea of murder being accepted but now using detail to show the way the characters act. Through the use of characterization, and detail the authors Connell, and Browning proved that in some minds people believe murder is acceptable. The idea of this satanic behavior from people is seen in the real world every single day. An act of this violence just recently occurred in the las vegas shooting, where a psychotic man decided to gun down a crowd at a concert from a hotel window. They still havent found out why he committed this random act of violence which just proves how delusional and crazy some people can be to the point where

Monday, December 16, 2019

The Effect of Transformation of pGLO in Bacteria Free Essays

Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab, bacteria was transformed by inserting DNA for Green Fluorescent Proteins. We will write a custom essay sample on The Effect of Transformation of pGLO in Bacteria or any similar topic only for you Order Now The DNA for these proteins were taken from bioluminescent jellyfish Aequorea victoria. One of the main lessons of the lab is learning of the use of ‘plasmids’. Plasmids are small pieces of DNA that usually code for one trait and are easily transferable between bacteria. This transfer of plasmids between bacteria is actually extremely helpful for them and are key in their survival. The plasmid that codes for the Green Fluorescent Proteins is accompanied with a gene for resistance to the antibiotic ampicillin. To ‘switch on’ the gene for fluorescence caused by the proteins, sugar arabinose must be added to the bacteria’s environment. If there is no sugar arabinose introduced to the plates, then the bacteria will appear white and will not glow, even if the gene for the proteins is successfully inserted. If the gene was successfully inserted and there is sugar arabinose present then the bacteria will glow a fluorescent green. The objectives for this lab is was to see the effects on bacteria in four different cases. The first case is the effect on bacteria when the gene for pGLO is introduced with LB (a ‘broth’ like substance that bacteria feed off of) and ampacillin. The second case is the effect on bacteria when the gene for pGLO is introduced with LB, ampacillin, and sugar arabinose. The third case is the effect on bacteria when no gene for pGLO is introduced, but LB and ampacillin is still introduced, The fourth case is the effect on bacteria when no gene for pGLO is introduced, but bacteria is still placed in a LB enriched environment. The hypothesis for the first plate is that bacteria will grow, however it will not glow even though the pGLO gene is introduced because there is no arabinose to effectively activate the gene. The bacteria will still grow although the ampacillin (which normally kills bacteria) is present because the pGLO gene also acts as a resistant to antibiotics. The hypothesis for the second plate is that bacteria will grow and glow because the gene for pGLO is introduced with sugar arabinose to effectively turn it on. The bacteria will also not die although ampacillin is present because, alike to the first plate, the pGLO assists the bacteria in becoming resistant to antibiotics. The hypothesis for the third plate is that no bacteria will grow at all because it is an ampacillin enriched environment with no pGLO gene to help the bacteria become resistant to the antibiotic. The hypothesis for the fourth plate is that the bacteria will grow normally because although there is no pGLO gene introduced, there is also no antibiotic to prevent the bacteria from growing. (AP Biology Development Committee, 2012) Data/Results: ? Figure 1. Recorded results from observing the transformed bacteria under light and in darkness After proper incubation time, we took the plates and viewed them with the lights on and then turned the lights off to see if any of the plates had colonies that glowed. As seen in Figure 1, the first plate produced some bacteria (one colony) and did not glow. The second plate produced a decent amount of bacteria (eight colonies) and ended up glowing. The third plate did not produce any bacteria at all leaving it impossible to see if anything glowed or not. The fourth plate produced the most amount of bacteria (ten colonies) and did not glow. Conclusion: All four of our hypotheses were correct after reviewing the results. The first plate, that consisted of bacteria with pGLO in an environment of LB and ampacillin, produced colonies however they did not glow due to the fact that there was nothing to turn on the pGLO gene. There needed to be arabinose in the environment for the gene to be expressed and since there was not there was no glow. The second plate, that consisted of bacteria with pGLO in an environment of LB, ampacillin, and arabinose, produced a fair amount of colonies that did end up glowing. The bacteria glowed because the pGLO was successfully inserted and transformed and had the arabinose to express the gene. The third plate, that consisted of bacteria without pGLO in an environment of LB and ampacillin, did not produce any bacteria. This outcome was due to the fact that ampacillin kills bacteria and there was no pGLO gene to help the bacteria become resistant to the antibiotic. The fourth plate, that consisted of bacteria without pGLO in an environment of just LB, produced the most amount of bacteria because although it did not have the pGLO gene to prevent antibiotics from killing the bacteria, there were no antibiotics to have to account for. It makes sense that the fourth plate produced the most bacteria because although in both plates one and two there was pGLO to prevent the ampacillin from killing the bacteria, not all of the bacteria were likely to go through transformation correctly and therefore not all of the bacteria had the pGLO ultimately resulting in the termination of a lot of potential bacteria colonies. (AP Biology Development Committee, 2012) How to cite The Effect of Transformation of pGLO in Bacteria, Papers

Sunday, December 8, 2019

Nonverbal Communication And The Body Language Essay Example For Students

Nonverbal Communication And The Body Language Essay Nonverbal communication are the signals that we put off when expressing to another individual. This is suggested to as our body language. However, it is always helpful to have a beneficial communication as a foundation acceptable to have an excellent relationship, either personal or professional. This subject indeed fascinated me, so I chose to research all that I could on this topic. I believe it’s a useful factor for each of us to recognize more about our nonverbal communication, in turn to have an improved understanding with those around us. Nonverbal communication is as worthy then what you are essentially speaking, therefore there are various distinctive categories of body language that an individual might interpret as somewhat otherwise. First point that I would like to discuss is nonverbal communication skills in a relationship. After all you’re nonverbal communication can furthermore play a vast amount in your relationship, as well. However, altogether your nonverbal communication is associated with speaking, and importantly listening too. Nonetheless once you can perfect your listening skills, then you have healthier responding skills. Therefore, furthering these skills, and your awareness of body language, also other nonverbal skills, everyone around you can benefit from you improving your communication skills. However, you should be more aware of the distinctive signs from your partner, otherwise they could go unnoticed. Although, if you can communicate effectively your overall happiness is increased, therefore improving your interpretation of your partners nonverbal communication skills. This will expand your emotional intelligence. Although, this can have a massive impact on any relationship. Furthe. . ing fingers, or fidgeting, could mean the person is impatient, or frustrated. Furthermore, you have a social distance mainly with friends; then you have a public distance with co-workers; finally a personal distance which is with couples.Nonverbal communication is as worthy then what you are essentially speaking, therefore there are various distinctive categories of body language that an individual might interpret as somewhat otherwise. I had no indication that your nonverbal was more than just how you held your hands, or the expression on your face. We are always being analyzed every time we interact with another individual. However, being aware of your nonverbal communication, you are more mindful of your emotional needs. When observing others pay attention to inconsistencies, look at nonverbal communication signals as a group, and trust your instincts.